Advanced post-synthesis process in oligos production

Oligos are produced through a solid-phase synthesis which offers an elevated yield of purified product which is then applied in the polymerase chain reaction (PCR), DNA sequencing, library construction and artificial gene synthesis. In order to be used in downstream applications, the oligonucleotide must be cleaved from the solid support and the protecting groups which need to be removed.

wavePREP offers a new approach to the post-synthesis process by providing cleavage and deprotection of oligos within a single step and by removing evaporation.

  • SUPERIOR PRODUCT YIELDS
  • FAST & AUTOMATED
  • IMPROVED SAFETY
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CLEAVAGE AND DEPROTECTION INFO A SINGLE STEP

At the completion of the synthesis process, the oligos have to be removed from the solid support and subsequently deprotected. These manual steps have a direct impact on the final yield, as well as on the productivity and on the lab’s workflow, due to the fact that their completion takes several hours. These steps are usually carried out by immersing the plates into ammonia solutions or other reagents, thus requiring an evaporation step and, therefore, creating unpleasant lab conditions.
wavePREP offers a new approach to the post-synthesis process by providing cleavage and deprotection of oligos within a single step and by removing evaporation.
In wavePREP, liquid reagents turn into gas, cleaving and deprotecting oligos simultaneously. Reaction conditions are accurately controlled to ensure complete process control and to maximize the yield. Once the procedure has finished, oligonucleotides can be directly desalted, for example with acetonitrile, and eluted from the solid support with the appropriate buffer solution without further steps.

CLEAVAGE AND DEPROTECTION INFO A SINGLE STEP

At the completion of the synthesis process, the oligos have to be removed from the solid support and subsequently deprotected. These manual steps have a direct impact on the final yield, as well as on the productivity and on the lab’s workflow, due to the fact that their completion takes several hours. These steps are usually carried out by immersing the plates into ammonia solutions or other reagents, thus requiring an evaporation step and, therefore, creating unpleasant lab conditions.
wavePREP offers a new approach to the post-synthesis process by providing cleavage and deprotection of oligos within a single step and by removing evaporation.
In wavePREP, liquid reagents turn into gas, cleaving and deprotecting oligos simultaneously. Reaction conditions are accurately controlled to ensure complete process control and to maximize the yield. Once the procedure has finished, oligonucleotides can be directly desalted, for example with acetonitrile, and eluted from the solid support with the appropriate buffer solution without further steps.
fast

FAST
AND AUTOMATED

wavePREP simultaneously performs cleavage and deprotection of oligos in only 90 minutes, saving up to 95% of the processing time. The lab workflow becomes more efficient as most of the steps are automated and software controlled, such as the lowering of the deepwell plates into the reactor, reagent addition, draining and venting.
INCREASED BREWING YIELDS

HIGH
PRODUCTIVITY

The shorter processing time, along with greater yields, leads to superior productivity. To match every need, wavePREP can be used either with deepwell plates, glassware, or columns. The flexible configurations and large reactor enable to process up to 6 deepwell plates (96 positions each) in only 90 minutes,
EASIER BLENDING AND FLAVOR ENRICHMENT

SUPERIOR
SAFETY

wavePREP has an intrinsic operation safety thanks to the ruggedness of the stainless-steel reactor and the highest manufacturing standards. The workflow of the system with the automated pump removes the handling of chemicals and any exposure whilst ensuring a fully controlled process throughout the temperature and pressure sensors.

Milestone

Established in 1988, Milestone is headquartered in Italy with R&D and manufacturing centre in Switzerland and offices in the United States, China, Japan and Korea. We operate worldwide through a network of exclusive distributors which provide premium application and service support to all our customers. Milestone’s mission is to offer the most advanced instrumentation for sample preparation and to address the named challenges in this sector.
Our industry-leading technology, in combination with fast and responsive service and application support, allows Milestone to achieve our goal of giving you the highest return on investment possible.