At the completion of the synthesis process, the oligos have to be removed from the solid support and subsequently deprotected. These manual steps have a direct impact on the final yield, as well as on the productivity and on the lab’s workflow, due to the fact that their completion takes several hours. These steps are usually carried out by immersing the plates into ammonia solutions or other reagents, thus requiring an evaporation step and, therefore, creating unpleasant lab conditions.
wavePREP offers a new approach to the post-synthesis process by providing cleavage and deprotection of oligos within a single step and by removing evaporation.
In wavePREP, liquid reagents turn into gas, cleaving and deprotecting oligos simultaneously. Reaction conditions are accurately controlled to ensure complete process control and to maximize the yield. Once the procedure has finished, oligonucleotides can be directly desalted, for example with acetonitrile, and eluted from the solid support with the appropriate buffer solution without further steps.