Advanced post-synthesis processes in oligos production

Oligos are synthesized via solid-phase synthesis method and find applications in various fields, including the PCR tests for viruses and pathogens detection, DNA sequencing artificial gene synthesis, rare diseases therapeutics, vaccines and frontier biomedical solutions. However, before these oligonucleotides can be used in downstream applications, they must be cleaved from the solid support and have their protecting groups removed.

wavePREP offers a new approach to the post-processing cleavage and deprotection (C&D) steps in oligonucleotide production. Combining cleavage and deprotection into a single step, wavePREP eliminates the need for evaporation and ensuring a superior workflow.

  • FAST & AUTOMATED
  • HIGH PRODUCTIVITY
  • PRODUCT YIELDS
  • SUPERIOR SAFETY
wavePREP

AUTOMATED LOADING/ DRAINING STATION

The system features a dedicated pump designed for the automated addition and drainage of reagents. This eliminates the need for direct operator exposure to chemicals, as the process is controlled by software and performed automatically.

HOLDERS

Available for different supports such as well plates and columns, the holders offer maximum flexibility to meet any requirement.

TEMPERATURE & PRESSURE SENSOR

The yield of oligos production greatly depends on the cleavage and deprotection processes. The precise temperature and pressure sensors ensure optimal control of the reaction procedure, resulting in maximized yields.

STAINLESS-STEEL CHAMBER

The reactions occur within a sealed stainlesssteel reactor, providing secure handling of pressure throughout the process.

USER INTERFACE

The user interface allows for complete control and tracking of all reaction parameters and functionalities. It is designed in full compliance with GMP regulations, ensuring adherence to quality standards.
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CLEAVAGE AND DEPROTECTION INTO A SINGLE STEP

At the completion of the synthesis process, the oligos have to be removed from the solid support and subsequently deprotected. These manual steps have a direct impact on the final yield, as well as on the productivity and on the lab’s workflow, due to the fact that their completion takes several hours. These steps are usually carried out by immersing the plates into ammonia solutions or other reagents, thus requiring an evaporation step and, therefore, creating unpleasant lab conditions.
wavePREP offers a new approach to the post-synthesis process by providing cleavage and deprotection of oligos within a single step and by removing evaporation.
In wavePREP, liquid reagents turn into gas, cleaving and deprotecting oligos simultaneously. Reaction conditions are accurately controlled to ensure complete process control and to maximize the yield. Once the procedure has finished, oligonucleotides can be directly desalted, for example with acetonitrile, and eluted from the solid support with the appropriate buffer solution without further steps.

CLEAVAGE AND DEPROTECTION INFO A SINGLE STEP

At the completion of the synthesis process, the oligos have to be removed from the solid support and subsequently deprotected. These manual steps have a direct impact on the final yield, as well as on the productivity and on the lab’s workflow, due to the fact that their completion takes several hours. These steps are usually carried out by immersing the plates into ammonia solutions or other reagents, thus requiring an evaporation step and, therefore, creating unpleasant lab conditions.
wavePREP offers a new approach to the post-synthesis process by providing cleavage and deprotection of oligos within a single step and by removing evaporation.
In wavePREP, liquid reagents turn into gas, cleaving and deprotecting oligos simultaneously. Reaction conditions are accurately controlled to ensure complete process control and to maximize the yield. Once the procedure has finished, oligonucleotides can be directly desalted, for example with acetonitrile, and eluted from the solid support with the appropriate buffer solution without further steps.

Principle of operation

WavePREP offers a single-step approach to cleavage and deprotection, eliminating the need for evaporation. The reaction occurs by heating the cleavage and deprotection reagents using microwave technology, causing its transition to the gas phase. Precise control over the temperature and pressure of both the vapor and liquid phases ensures optimal yield and safe operation. Consequently, at the end of the process, there is no requirement for evaporation, and the oligonucleotides can be directly eluted for following work-up. This streamlined operation saves time, improves efficiency and enhances the overall workflow
  • STEP 1

    Automatic reactor chamber closure.
  • STEP 2

    Reagents are automatically pumped into the chamber.
    Automated purging to eliminate any reagents.
  • STEP 3

    Microwaves emission starts, reagents vapors are generated.
  • STEP 4

    Rapid cooling, venting, and removal of residual reagents.
  • STEP 5

    Automated purging to eliminate any reagents residue.
  • STEP 6

    Opening of clamps and automatic raising of sample holders.
  • STEP 1
  • STEP 2
  • STEP 3
  • STEP 4
  • STEP 5
  • STEP 6
High versatility for an easy implementation

High versatility for
an easy implementation

Many times, the use of customized synthesizers can restrict the integration of other technologies into the process. However, WavePREP is specifically designed to offer maximum flexibility and compatibility with virtually any synthesizers. This allows for the utilization of well plates and columns, facilitating an easy implementation process. Additionally, customized holders can be created to accommodate specific requirements of both research and production labs, suitable for nmol up to mmol scales.
High versatility for an easy implementation

Smart user interface

The wavePREP is operated through a compact terminal equipped with the user-friendly and icondriven easyCONTROL software. This intuitive software allows users to control and monitor all reaction parameters throughout the entire run, ensuring reliable and replicable operation and high safety standards.
The system includes multiple sensors that control and monitor crucial parameters such as the temperature of the liquid and vapor phases, pressure, microwave power, and time. Additionally, the software enables automation of several steps, including reagent loading, cooling, chamber venting, reagent removal, and pressure line flushing.

Validation Package

Compliance with regulations and data traceability requirements is of utmost importance in modern laboratories. Milestone's operating software, easyCONTROL, fully complies with FDA regulation 21 CFR part 11. Additionally, our comprehensive, including IQ and OQ qualifications, provides access to our factory-trained engineers and specialists during the qualification process. This facilitates a swift implementation of the wavePREP system in complete accordance with Good Manufacturing Practice (GMP) guidelines.
Fast and Automated

FAST
AND AUTOMATED

wavePREP enables the simultaneous cleavage and deprotection of oligos in just up to 90 minutes, resulting in a remarkable time-saving of up to 95% compared to traditional methods. The system significantly enhances lab workflow efficiency by automating and softwarecontrolling various steps, including the lowering of well plates into the reactor, reagent addition, chamber draining, and venting. With wavePREP, laboratories can achieve faster and more streamlined processes, optimizing productivity
High Productivity

HIGH
PRODUCTIVITY

The combination of shorter processing time and higher yields achieved with wavePREP results in superior productivity. This versatile system can be used with well plates OR columns to accommodate various needs. With its flexible configurations and large reactor capacity, wavePREP can process up to 6 well plates in just 90 minutes. Furthermore, there is no need to perform an evaporation step after wavePREP C&D, shorteing even more the overall process lenght.
Product Yields

PRODUCT
YIELDS

The conventional postsynthesis approach involves multiple critical steps to achieve superior yields. However, the wavePREP approach eliminates some of these crucial steps and operates within a controlled environment. By doing so, it ensures significantly higher yields compared to traditional approaches. With wavePREP, laboratories can expect improved efficiency and increased productivity, leading to enhanced overall product yields.
Superior Safety

SUPERIOR
SAFETY

wavePREP prioritizes operational safety through its robust stainless-steel reactor and adherence to the highest manufacturing standards. The system's workflow, facilitated by the automated pump, eliminates the need for direct handling of chemicals, minimizing operator exposure. The temperature and pressure sensors ensure a fully controlled process, further enhancing safety measures. An added benefit is the elimination of the reagents evaporation step, leading to an improvement of the work environment.

Milestone

Established in 1988, Milestone is headquartered in Italy with R&D and manufacturing centre in Switzerland and offices in the United States, China, Japan and Korea. We operate worldwide through a network of exclusive distributors which provide premium application and service support to all our customers. Milestone’s mission is to offer the most advanced instrumentation for sample preparation and to address the named challenges in this sector.
Our industry-leading technology, in combination with fast and responsive service and application support, allows Milestone to achieve our goal of giving you the highest return on investment possible.