Advanced post-synthesis processes in oligos production
wavePREP offers a new approach to the post-processing cleavage and deprotection (C&D) steps in oligonucleotide production. Combining cleavage and deprotection into a single step, wavePREP eliminates the need for evaporation and ensuring a superior workflow.
- FAST & AUTOMATED
- HIGH PRODUCTIVITY
- PRODUCT YIELDS
- SUPERIOR SAFETY
AUTOMATED LOADING/ DRAINING STATION
The system features a dedicated pump
designed for the automated addition and
drainage of reagents. This eliminates the need
for direct operator exposure to chemicals,
as the process is controlled by software and
performed automatically.
HOLDERS
Available for different supports such as well
plates and columns, the holders offer maximum
flexibility to meet any requirement.
TEMPERATURE & PRESSURE SENSOR
The yield of oligos production greatly depends
on the cleavage and deprotection processes.
The precise temperature and pressure
sensors ensure optimal control of the reaction
procedure, resulting in maximized yields.
STAINLESS-STEEL CHAMBER
The reactions occur within a sealed stainlesssteel
reactor, providing secure handling of
pressure throughout the process.
USER INTERFACE
The user interface allows for complete control
and tracking of all reaction parameters and
functionalities. It is designed in full compliance
with GMP regulations, ensuring adherence to
quality standards.
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CLEAVAGE AND DEPROTECTION INFO A SINGLE STEP
At the completion of the synthesis process, the oligos have to be removed from the solid support and
subsequently deprotected. These manual steps have a direct impact on the final yield, as well as on the
productivity and on the lab’s workflow, due to the fact that their completion takes several hours. These steps
are usually carried out by immersing the plates into ammonia solutions or other reagents, thus requiring
an evaporation step and, therefore, creating unpleasant lab conditions.
wavePREP offers a new approach to the post-synthesis process by providing cleavage and deprotection of oligos within a single step and by removing evaporation.
In wavePREP, liquid reagents turn into gas, cleaving and deprotecting oligos simultaneously. Reaction conditions are accurately controlled to ensure complete process control and to maximize the yield. Once the procedure has finished, oligonucleotides can be directly desalted, for example with acetonitrile, and eluted from the solid support with the appropriate buffer solution without further steps.
wavePREP offers a new approach to the post-synthesis process by providing cleavage and deprotection of oligos within a single step and by removing evaporation.
In wavePREP, liquid reagents turn into gas, cleaving and deprotecting oligos simultaneously. Reaction conditions are accurately controlled to ensure complete process control and to maximize the yield. Once the procedure has finished, oligonucleotides can be directly desalted, for example with acetonitrile, and eluted from the solid support with the appropriate buffer solution without further steps.
CLEAVAGE AND DEPROTECTION INFO A SINGLE STEP
At the completion of the synthesis process, the oligos have to be removed from the solid support and
subsequently deprotected. These manual steps have a direct impact on the final yield, as well as on the
productivity and on the lab’s workflow, due to the fact that their completion takes several hours. These steps
are usually carried out by immersing the plates into ammonia solutions or other reagents, thus requiring
an evaporation step and, therefore, creating unpleasant lab conditions.
wavePREP offers a new approach to the post-synthesis process by providing cleavage and deprotection of oligos within a single step and by removing evaporation.
In wavePREP, liquid reagents turn into gas, cleaving and deprotecting oligos simultaneously. Reaction conditions are accurately controlled to ensure complete process control and to maximize the yield. Once the procedure has finished, oligonucleotides can be directly desalted, for example with acetonitrile, and eluted from the solid support with the appropriate buffer solution without further steps.
wavePREP offers a new approach to the post-synthesis process by providing cleavage and deprotection of oligos within a single step and by removing evaporation.
In wavePREP, liquid reagents turn into gas, cleaving and deprotecting oligos simultaneously. Reaction conditions are accurately controlled to ensure complete process control and to maximize the yield. Once the procedure has finished, oligonucleotides can be directly desalted, for example with acetonitrile, and eluted from the solid support with the appropriate buffer solution without further steps.
Principle of operation
WavePREP offers a single-step approach to cleavage and deprotection, eliminating the need for evaporation. The reaction occurs by heating the cleavage and deprotection reagents using microwave technology, causing its transition to the gas phase. Precise control over the temperature and pressure of both the vapor and liquid phases ensures optimal yield and safe operation. Consequently, at the end of the process, there is no requirement for evaporation, and the oligonucleotides can be directly eluted for following work-up. This streamlined operation saves time, improves efficiency and enhances the overall workflow
-
STEP 1
Automatic reactor chamber closure. -
STEP 2
Reagents are automatically pumped into the chamber.
Automated purging to eliminate any reagents. -
STEP 3
Microwaves emission starts, reagents vapors are generated. -
STEP 4
Rapid cooling, venting, and removal of residual reagents. -
STEP 5
Automated purging to eliminate any reagents residue. -
STEP 6
Opening of clamps and automatic raising of sample holders.
High versatility for
an easy implementation
Many times, the use of customized synthesizers can restrict the integration of other technologies into the process.
However, WavePREP is specifically designed to offer maximum flexibility and compatibility with virtually any
synthesizers. This allows for the utilization of well plates and columns, facilitating an easy implementation process.
Additionally, customized holders can be created to accommodate specific requirements of both research and
production labs, suitable for nmol up to mmol scales.
Smart user interface
The wavePREP is operated through a compact
terminal equipped with the user-friendly and icondriven easyCONTROL software. This intuitive
software allows users to control and monitor all
reaction parameters throughout the entire run,
ensuring reliable and replicable operation and
high safety standards.
The system includes multiple sensors that control and monitor crucial parameters such as the temperature of the liquid and vapor phases, pressure, microwave power, and time. Additionally, the software enables automation of several steps, including reagent loading, cooling, chamber venting, reagent removal, and pressure line flushing.
The system includes multiple sensors that control and monitor crucial parameters such as the temperature of the liquid and vapor phases, pressure, microwave power, and time. Additionally, the software enables automation of several steps, including reagent loading, cooling, chamber venting, reagent removal, and pressure line flushing.
Validation Package
Compliance with regulations and data traceability requirements is of utmost importance in modern laboratories. Milestone's operating software, easyCONTROL, fully complies with FDA regulation 21 CFR part 11. Additionally, our comprehensive, including IQ and OQ qualifications, provides access to our factory-trained engineers and specialists during the qualification process. This facilitates a swift implementation of the wavePREP system in complete accordance with Good Manufacturing Practice (GMP) guidelines.
FAST
AND AUTOMATED
wavePREP enables the
simultaneous cleavage and
deprotection of oligos in just
up to 90 minutes, resulting in
a remarkable time-saving of
up to 95% compared to traditional methods.
The system significantly enhances lab workflow
efficiency by automating and softwarecontrolling
various steps, including the lowering
of well plates into the reactor, reagent addition,
chamber draining, and venting. With wavePREP,
laboratories can achieve faster and more
streamlined processes, optimizing productivity
HIGH
PRODUCTIVITY
The combination of shorter
processing time and higher
yields achieved with wavePREP
results in superior productivity.
This versatile system can be used
with well plates OR columns to accommodate
various needs. With its flexible configurations and
large reactor capacity, wavePREP can process up
to 6 well plates in just 90 minutes. Furthermore,
there is no need to perform an evaporation step
after wavePREP C&D, shorteing even more
the overall process lenght.
PRODUCT
YIELDS
The conventional postsynthesis
approach involves
multiple critical steps to achieve
superior yields. However, the
wavePREP approach eliminates
some of these crucial steps and operates within
a controlled environment. By doing so, it ensures
significantly higher yields compared to traditional
approaches. With wavePREP, laboratories
can expect improved efficiency and increased
productivity, leading to enhanced overall product
yields.
SUPERIOR
SAFETY
wavePREP prioritizes
operational safety through its
robust stainless-steel reactor
and adherence to the highest
manufacturing standards. The
system's workflow, facilitated by the automated
pump, eliminates the need for direct handling of
chemicals, minimizing operator exposure. The
temperature and pressure sensors ensure a fully
controlled process, further enhancing safety
measures. An added benefit is the elimination
of the reagents evaporation step, leading to an
improvement of the work environment.
Milestone Srl
Via Fatebenefratelli 1/5 - 24010 Sorisole (BG) - Italy
Phone: +39 035.573857 | Fax: +39 035.575498
Via Fatebenefratelli 1/5 - 24010 Sorisole (BG) - Italy
Phone: +39 035.573857 | Fax: +39 035.575498
Milestone Srl
Via Fatebenefratelli 1/5 - 24010 Sorisole (BG) - Italy
Phone: +39 035.573857 | Fax: +39 035.575498
Via Fatebenefratelli 1/5 - 24010 Sorisole (BG) - Italy
Phone: +39 035.573857 | Fax: +39 035.575498