Total fat determination in food and feed matrices is a routine analysis for several testing and food processor labs, as it is part of labelling process and QC testing. Nowadays, the process still relies on outdated and matrix-dependent methods which use obsolete and time-consuming technologies. In the ETHOS X hydrolysis and extraction are done in a single step and in less time, ensuring higher productivity and better workflow. The ETHOS X method for total fat determination is applicable to any matrix; enables to process 45 sample a day, ensure lower cost per sample, offers complete fat determination and it is safe and compact.